KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias. This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.
In addition to the standard library amplification formulation, KAPA HiFi is available in a unique real-time formulation, for applications that demand precise control over library amplification. A uracil-tolerant variant, KAPA HiFi Uracil+ is also available for the high-efficiency, high-fidelity, low-bias amplification of libraries constructed from bisulfite-treated DNA.
Achieve the lowest amplification bias and duplication rates
Improve coverage of GC- and AT-rich regions
Exercise precise control over library amplification
Improved amplification of GC- and AT-rich genomic regions
For life science research only.
Not for use in diagnostic procedures.
Data on file.