KAPA HyperPlus Kits

KAPA HyperPlus Kits

NGS library preparation. Evolved.

The KAPA HyperPlus Kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube. Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows. 


Your benefit


Tunable and reproducible ­fragmentation

  • Adjust library insert sizes from 150 – 800 bp by varying fragmentation time
  • Reproducible insert sizes across a range of GC content and DNA input amounts


Industry leading library yields…

  • Routinely achieve conversion rates >80 % from ≥100 ng input*
  • Superior performance across a range of DNA input amounts*
  • High library yields enable PCR-free ­workflows from as little as 50 ng starting material*

…can enable superior sequencing results

  • High conversion rates results in fewer amplification cycles and lower duplication rates
  • Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage


Minimal sequence coverage bias

  • Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods*
  • Equivalent performance to mechanical shearing*
  • Less bias leads to more uniform sequencing coverage and reduced sequencing costs*


Product characteristics


  • DNA fragmentation and library prep in 2.5 hours*
  • Flexible DNA sample input from 1 ng – 1 µg*
  • Reduced bias and maximize sequence coverage*
  • PCR-free workflows



For Research Use Only. Not for use in diagnostic procedures.
*Data on file.