KAPA Pure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA and RNA next-generation sequencing library construction workflows.
Seamless integration into NGS workflows
Tunable and highly reproducible size selection
KAPA single-indexed adapter kits contain high-quality, ready-to-use adapters for Illumina® library construction. Each adapter includes a single, 6-nt index (barcode) for multiplexed sequencing applications. KAPA Single-Indexed Adapters are available in two concentrations, and are compatible with all KAPA library preparation kits for DNA and RNA sequencing applications.
KAPA Single-Indexed Adapters undergo extensive qPCR- and sequencing-based functional and QC testing to confirm:
Recommended Adapter Concentration by Input
|Kit||30 μM||1.5 μM|
|KAPA HyperPlus and Hyper Prep Kits||≥5 ng – 1 μg||<5 ng|
|KAPA HTP or LTP “with-bead” Library Preparation Kits||≥100 ng*||≤100 ng*|
|KAPA Library Preparation Kits||
(1 – 5 μg)
|KAPA Stranded RNA-Seq Kits with RiboErase||not recommended||all Imputs|
|KAPA Stranded RNA-Seq and mRNA-Seq Kits||not recommended||all inputs and workflows|
* For 100 ng input the recommended adapter concentration depends on average insert size
For life science research only.
Not for use in diagnostic procedures.
Data on file.