KAPA Stranded RNA-Seq with RiboErase

KAPA Stranded RNA-Seq with RiboErase

Evolved to focus

The KAPA Stranded RNA-Seq Kit with ­RiboErase offers a high-quality, comprehensive solution for transcriptome sequencing. By utilizing a targeted enzymatic method for depletion, our workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPA HiFi for high-efficiency and ­low-bias library amplification, and include a streamlined, “with-bead” protocol.

 

Your benefit

 

  • Up to 99.98 % rRNA depletion 
  • Flexible input of 100 ng – 1 μg total RNA for human, mouse, or rat species 
  • Robust and reproducible results across various input amounts 
  • An automation-friendly workflow 

 

Product characteristics

 

Industry-leading rRNA depletion 

  • Very good rRNA depletion from high-quality and FFPE samples 
  • More economical NGS sequencing due to decreased rRNA reads, providing deeper sequencing of transcripts of interest 

 

Maximum coverage uniformity 

  • Uniform distribution of reads over each transcript 
  • Minimal 5’ – 3’ bias across transcripts 

 

High-quality sequencing data 

  • Detection of more genes and unique ­transcripts 
  • Accurate and clear identification of splice sites and alternative gene splicing 
  • Improved coverage enabling better detection of difficult and GC-rich transcripts 

 

Highly reproducible sequencing results 

  • High correlation even between different testing conditions 
  • Low sample-to-sample variation for more reliable results